Services and pricing

Services

The Fisabio-Public Health Sequencing and Bioinformatics Service provides counselling on the planning of studies, as well as the exploitation of massive sequencing data. Our mission is to help the user take full advantage of these technologies and withdraw as much information as possible from the sequencing data. We also offer different pipelines for the analyses, and guidance for the interpretation of results.

Active sequencing protocols

FISABIO's sequencing service offers the possibility of multiplexing (joining samples), according to the coverage required by the user.

  • Amplicon Sequencing
  • Complete genome sequencing
  • Complete genome resequencing
  • Transcriptome sequencing
  • Metagenome sequencing
  • Metatranscriptome sequencing
  • Small RNAs sequencing
  • Gene panels

Bioinformatics

FISABIO's bioinformatics service offers standardized pipelines for the analysis of sequencing data. We also offer the possibility of writing ad hoc scripts and pipelines, elaborated specifically for the necessities of the users. These analyses include (but are not limited to):

  • Bacterial taxonomic annotation (16s rDNA amplicon sequencing)
  • Fungal taxonomic annotation (ITS amplicon sequencing)
  • Diversity and comparative analysis of microbial and/or fungal communities (16S/ITS)
  • Genome/transcriptome and metagenome/metatranscriptome assembly and annotation
  • Variant calling in resequencing studies
  • Comparative analysis between multiple genomes to define snps/indels profiles and differential genes, pangenomes

Counseling

The Fisabio Sequencing and Bioinformatics Service offers counseling based on the experience acquired in its trajectory: from the first second generation sequencers (Roche FLX + 454), through the evolution of Illumina sequencers, to the new technologies of third generation (Oxford Nanopore Technologies and PacBio). The main aspects are:

  • Support in the experimental design of research projects where massive sequencing and bioinformatics support is needed.
  • Direction and management of start-up projects for new sequencing and bioinformatics laboratories.

Forensic genetics

Forensic Genetics is a specialty of Genetics that includes a set of knowledge necessary to solve certain legal problems. The types of expertise most requested from the Forensic Genetics laboratory by the courts are cases of biological investigation of paternity, biological criminalistics expertise (study of biological vestiges of criminal interest such as blood stains, sperm, hair, etc.) and, finally identification problems.

At the Fisabio Sequencing and Bioinformatics Service we offer forensic genetics services based on massive second-generation sequencing. We have a MiSeq FGx sequencer developed and validated for forensic genomics that allows obtaining genetic profiles that include more than 200 markers, including autosomal SNPs, autosomal and sexual STRs (Y-STR and X-STR) and mitochondrial DNA genetic profiles. .

We are also the laboratory designated by the Ministry of Justice to carry out DNA studies on historical memory issues in the Valencian Community.

We offer the following services always within the framework of research projects and never to individuals:

  • DNA extraction from reference samples of blood or saliva from different supports (buccal swab or tissue) *.
  • Obtaining genetic DNA profiles using Illumina technology (MiSeq FGx)
  • Mitochondrial DNA Libraries (ForenSeq mtDNA Control Region Kit) *.
  • Identification and kinship analysis *.
  • Bone DNA extraction, quantification and libraries *.

Note: Services that appear with the symbol (*) are not included in the scope of ISO/IEC 17025 accreditation.

For more information, do not hesitate to contact us by phone 680533221 (via call or WhatsApp) or by writing an email to geneticaforense@fisabio.es.

Quality

In the Testing Laboratory we have a Quality Management System (QMS) in accordance with the UNE-EN ISO/IEC 17025 standard, to ensure that the activity carried out and the technical competence in carrying out tests is carried out with the maximum quality guarantees.

Therefore, our Sequencing and Bioinformatics Service Testing Laboratory is accredited by ENAC with accreditation nº1515/LE2785.

Our accreditation ranges from DNA extracts to obtaining human genetic profiles using the latest NGS massive sequencing technology.

ENAC-ISO17025

Certifications

Clinical Laboratory Certification (UNE-EN ISO/IEC 17025)

Analysis of DNA extracts from reference blood and saliva swab samples and determination of genetic profiles using NGS.

Intercomparison exercises

In order to offer a quality and updated service, we periodically subject all our processes (both those that are included in the scope of accreditation and those that are not) to intercomparison exercises with external international entities. These controls guarantee the assurance of the quality of the results obtained over time, as well as the sharing of knowledge and innovative concepts between specialists in the world of forensic genetics.

  • Internacional Society of Forensic Genetics. Intercomparison exercises GHEP-ISFG
  • The GEDNAP (German DNA profiling group) proficiency testing system
Imagen 2
Imagen 3

Quality and impartiality policy

The implementation of a quality and impartiality policy in accordance with ISO/IEC 17025:2017 is essential to establish and maintain confidence in laboratory results, customer satisfaction and regulatory compliance, thus guaranteeing excellence in technical competence.

Pricing

Fisabio’s platforms and services unit

Download Pricing

Illumina sequencing

  • Illumina amplicon libraries and sequencing 2x300bp
  • Amplicon libraries for illumina without sequencing
  • Illumina amplicon libraries and sequencing (high priority) ≤10 samples 2x250bp
  • Illumina amplicon libraries and sequencing (high priority) 2x300bp
  • Illumina metagenome libraries and sequencing 6 Gb per sample (2x150bp)
  • Illumina metagenome libraries and sequencing 15 Gb per sample (2x150bp)
  • Illumina genome libraries and sequencing <10Mb 0.5Gb per sample (2x150bp)
  • Illumina genome libraries and sequencing <10Mb 1Gb per sample (2x150bp) ≤ 25 samples
  • Illumina genome libraries and sequencing 25 samples
  • Genome/metagenome libraries for illumina without sequencing
  • Illumina RNA-seq/metatranscriptome libraries and sequencing 6Gb per sample (2x150bp)
  • Illumina RNA-seq/metatranscriptome libraries and sequencing 15Gb per sample (2x150bp)
  • Illumina RNA-seq/metatranscriptome libraries and sequencing (low input) 15Gb per sample (2x150bp)
  • Eukaryotic RNA-seq illumina libraries and sequencing
  • Illumina small RNA/microRNA libraries and sequencing 5 million reads per sample 1(x50pb)
  • NextSeq™ 1000/2000 P1 Reagents Run (100 cycles)
  • NextSeq™ 1000/2000 P1 Reagents Run (300 cycles)
  • NextSeq™ 1000/2000 P1 Reagents Run (600 cycles)
  • NextSeq™ 1000/2000 P2 Reagents Run (100 cycles)
  • NextSeq™ 1000/2000 P2 Reagents Run (200 cycles)
  • NextSeq™ 1000/2000 P2 Reagents Run (300 cycles)
  • NextSeq™ 1000/2000 P2 300M RGTSKit Run (600 cycles)
  • NextSeq™ 2000 P3 Reagents Run (100 Cycles)
  • NextSeq™ 2000 P3 Reagents Run (200 Cycles)
  • NextSeq™ 2000 P3 Reagents Run (300 Cycles)
  • NextSeq™ 2000 P4 Reagents Run (50 Cycles)
  • NextSeq™ 2000 P4 Reagents Run (100 Cycles)
  • NextSeq™ 2000 P4 Reagents Run (200 Cycles)
  • NextSeq™ 2000 P4 Reagents Run (300 Cycles)
  • Miseq nano 2x250bp Run
  • Miseq v3 2x300pb Run
  • Load a sequencer with client's sample and cartridges

PacBio sequencing

  • PacBio VEGA Run (50-60Gb)
  • Amplicon libraries up to 2Kb PACBIO and sequencing
  • Amplicon libraries up to 2Kb PacBio without sequencing
  • Genome libraries < 10Mb PACBIO or metagenomes without sequencing
  • Metagenomes for assembly (6Gb per sample)
  • Metagenomes for profiling (1Gb per sample)
  • Genome libraries < 10Mb and PacBio sequencing
  • Ultra-long amplicon libraries < 2kb and PacBio sequencing
  • Ultra-long amplicon libraries > 2kb without sequencing
  • Human Genome 20x
  • Human genome methylation profile
  • Eukaryotic genome 50Gb
  • Kinnex 16S VEGA amplicon libraries without sequencing
  • Kinnex 16S VEGA amplicon libraries and sequencing
  • Kinnex full-length RNA kit PACBIO libraries and sequencing (5 million reads)
  • Kinnex full-length RNA kit PACBIO libraries and sequencing (10 million reads)

Additional sample treatments

  • DNA/RNA extraction
  • DNA/RNA extraction with filtration for viral fraction
  • Fragment determination by TapeStation
  • Fragment determination by Fragment Analyzer
  • DNase treatment
  • RNase treatment
  • Quantification with Qubit

Nanopore sequencing

  • 16S amplicon libraries and nanopore sequencing
  • Custom amplicon libraries and nanopore sequencing ≤20
  • Custom amplicon libraries and nanopore sequencing >20
  • Custom amplicon libraries without sequencing
  • Extra-long amplicon libraries and nanopore sequencing
  • Genome libraries < 10Mb and nanopore sequencing
  • Plasmid libraries and nanopore sequencing
  • Load a nanopore flow cell

Bioinformatic analysis

  • Upstream taxonomic annotation of amplicons (16S, 18S, ITS)
  • Downstream comparative analysis of amplicons (16S, 18S, ITS)
  • Metagenome/metatranscriptome mapping
  • Mapping and differential analysis of metagenomes/metatranscriptomes
  • Metagenome/metatranscriptome assembly and annotation
  • Assembly, annotation, and differential analysis of metagenomes/metatranscriptomes
  • Bacterial and phage genome mapping
  • Automated assembly of genomes/metagenomes using PacBio/Nanopore/Illumina technology (contigs only)
  • Automated annotation of bacterial and phage genomes
  • Comparative analysis between bacterial genomes (<10Mb), pangenome (No assembly)
  • Identification of bacterial strains from their complete genome (No assembly)
  • Resistance/Virulence factor analysis in a bacterial genome (No assembly)
  • Bacterial genome characterization, annotation, and resistance/virulence factors (No assembly)
  • Viral genome mapping
  • Differential analysis of eukaryotic transcriptomes (without mapping)
  • Differential and functional analysis of eukaryotic transcriptomes (without mapping)
  • Differential analysis of bacterial transcriptomes (without mapping)
  • Differential and functional analysis of bacterial transcriptomes (without mapping)
  • Small RNA annotation and quantification
  • Differential analysis of small RNA (without annotation)
  • Design and/or implementation of custom gene panel analysis (consult)
  • Gene panel analysis
  • Mapping of complete human genomes and variant calling
  • Data submission (per data set)

Education

  • Customized course
  • Preparation of teaching materials for students