Module 1/ Introduction to Genomics for Microbiologists: from diagnostics applications to outbreak detection
Dates: June 22nd- June 26tt 2020
Organizer: Prof. Fernando González-Candelas (firstname.lastname@example.org)
The course is addressed to graduate students, professionals and practitioners of biology, biomedical research and the health sciences. Some background in microbiology, molecular biology, molecular evolution and phylogenetics will be advantageous, but it is not required. This course will introduce the methods and techniques used in the analysis of genome sequences obtained through high-throughput sequencing (HTS) technologies and their direct application in clinical and epidemiology problems. HTS are becoming the new gold standard in clinical microbiology laboratories for many different tests, including typing, identification of resistance and virulence factors, and surveillance of plasmids and other relevant genetic factors.
Molecular epidemiology is an emerging discipline in the interface between molecular biology, population and evolutionary genetics, and traditional epidemiology, interested in providing a perspective on population and epidemic processes from the perspective of the infectious organism. From the origin of new pathogens to the sources of outbreaks or more detailed surveillance, there is an increasing number of problems that can be tackled with molecular epidemiology approaches.
Practicing microbiologists and epidemiologists should become familiar with these new methodologies. This course provides an excellent opportunity for breaking the initial barriers, providing a head start for further advances.The course will consist of theoretical lectures, practical sessions with computers and seminars, with an emphasis on fundamental concepts and applications. Attendants are welcome to bring their own data.
Module costs: 350 euros
FGC-Fernando González-Candelas, PhD
FISABIO-Public Health Senior Scientist, Genomics and Health Area and Professor of Genetics, University of Valencia
MAB-María Alma Bracho Lapiedra PHD
FISABIO-Public Health Scientist, Genomics and Health Area
FXL-F. Xavier López Labrador, PHD
FISABIO-Public Health Senior Scientist, Genomics and Health Area
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AC-Álvaro Chiner Oms, PhD
Institute of Biomedicine-CSIC. TB Genomics Area
NGG-Neris García González, MSc Bioinformatics
Univ. Valencia-FISABIO Public Health Graduate student. Genomics and Health Area
BB-Beatriz Beamud Aranguren, MSc Bioinformatics
Univ. Valencia-FISABIO Public Health Graduate student.Genomics and Health Area
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Module program Summer School 2020:
Monday June 22nd: Introduction and Methodologies
09:00 – 11:00 Welcome and introduction to molecular epidemiology FGC.
11:30 - 13:30 Sequencing technologies for microbial genomics. FXL/BB.
13:00 - 13:30 Installation of Virtual Machines. MAB.
15:00 - 18:00 Practical session: Introduction to Bioinformatics. AC/MAB.
Tuesday June 23rd: Theoretical Foundations
09:00 - 11:00 Phylogenetic inference from molecular data. MAB.
11:30 - 13:30 Foundations of HTS analysis. NGG/BB.
15:00 - 18:00 Practical session: HTS I: managing output files. Analyses with reads. NGG/BB.
Wednesday June 24th: Analysis of Genetic Diversity
09:00 - 11:00 Bacterial genome evolution. FGC/NGG.
11:30 - 13:30 Molecular Epidemiology and Population Genetics of Viruses and Bacteria. FXL/FGC.
15:00 - 18:00 Practical session: HTS II: assembling and mapping. AC/NGG.
Thursday June 25th: Advanced Topics in Molecular Epidemiology
09:00 - 11:00 Virus surveillance using HTS. FXL.
11:30 - 13:30 Applications of HTS in Clinical Epidemiology. FGC/NGG.
15:00 - 18:00 Practical session: HTS III: variant calling, multiple sequence alignments. AC/NGG.
Friday June 26th: Seminars
09:00 - 12:00 Practical session: HTS IV: Phylogenetic trees from HTS data. MAB/NGG
12:30 - 14:30 Molecular Epidemiology Seminars – I. FXL/FGC.