|SUMMER SCHOOL - Module 1|
Module 1/ Preparation of Metagenomic and Amplicon Libraries for Illumina Platforms.
Dates: June 14th- June 16th 2017
Coordinator: Llúcia Martínez Priego (firstname.lastname@example.org)
The module will be practical and hands-on, and will teach participants to prepare two different libraries for Illumina platforms: 16S amplicon libraries for taxonomy analyses and whole genome amplification libraries for metagenomic analyses. Brief theoretical sessions and practical hands-on sessions will be taught by FISABIO Sequencing Service staff.
Module costs: 480 euros
Pre-registration (now open) until 15th of April 2017
Registration deadline 31st of May 2017
This module already reached the maximum number of participants but, go on filling the registration form to access the waiting list
Llúcia Martínez Priego PhD. Manager in the Sequencing Service of FISABIO-SP.
Wednesday June 14th:
10:00 – 10:30 Best practices in a NGS laboratory
11:00 – 12:00 DNA quantification and normalisation
12:00 – 13:00 16S amplicon PCR
14:30 – 16:30 DNA tagmentation and library clean up
16:30 – 17:30 Indexing
10:00 – 10:30 Bioanalyzer set up for library validation
11:00 – 11:30 Library quantification
11:30 – 13:00 Review of library validation results
14:30 – 16:30 Library normalization
16:30 – 17:30 Create Sample sheet for Illumina MiSeq RUN
11:00 – 13:30 Loading libreries for sequencing
13:30 – 15:00 Course feedback and wrap up