Contacto

 

 

Directora Summer School: 
M. Pilar Francino
 
Administrador Summer School:
Kevin Cifuentes
Telf.: 961 925 911

 

 
 

SUMMER SCHOOL - Module 1

 

 

 


Module 1/ Preparation of Metagenomic and Amplicon Libraries for Illumina Platforms.

Coordinator: Llúcia Martínez Priego (martinez_lucpri@gva.es)

NEW EDITION IN SEPTEMBER!

Dates: September 27th – September 29th 2017  

Pre-registration

The course is addressed to professionals of microbiology services in hospitals, NGS laboratory technicians and other professionals in microbiology, health sciences and related fields who are interested in learning new techniques to approach the study of microbiota.

The module will be practical and hands-on, and will teach participants to prepare two different libraries for Illumina platforms: 16S amplicon libraries for taxonomy analyses and whole genome amplification libraries for metagenomic analyses. Brief theoretical sessions and practical hands-on sessions will be taught by FISABIO Sequencing Service staff.

Official language: English or Spanish (depending of the student's origin)

Module costs: 480 euros


Instructors:
 

Llúcia Martínez Priego PhD. Manager in the Sequencing Service of FISABIO-SP.
Paula Corell Escuin. MS. Research Assistant in Sequencing Service of FISABIO-SP.

 

Llúcia Martínez Priego

PhD. Manager in the Sequencing Service of FISABIO-SP.

martinez_lucpri@gva.es

Paula Corell Escuin

MS. Research Assistant in Sequencing Service of FISABIO-SP.

corell_pau@gva.es




Course program:

Wednesday September 27th:


09:15 – 10:00 An Introduction to NGS library preparation
        Llúcia Martínez Priego

10:00 – 10:30 Best practices in a NGS laboratory
        Paula Corell Escuin

Break

11:00 – 12:00 DNA quantification and normalisation
        Llúcia and Paula

12:00 – 13:00 16S amplicon PCR
        Llúcia and Paula

Lunch

14:30 – 16:30 DNA tagmentation and library clean up
        Llúcia and Paula

16:30 – 17:30 Indexing
        Llúcia and Paula


Thursday September 28th:

09:00 – 10:00 Indexed library clean up
        Llúcia and Paula

10:00 – 10:30 Bioanalyzer set up for library validation    
        Llúcia and Paula

Break

11:00 – 11:30 Library quantification
        Llúcia and Paula

11:30 – 13:00 Review of library validation results
        Llúcia Martínez Priego

Lunch

14:30 – 16:30 Library normalization
        Llúcia and Paula

16:30 – 17:30 Create Sample sheet for Illumina MiSeq RUN
        Llúcia Martínez Priego


Friday September 29th:

09:00 – 10:45 Preparing the MiSeq and the loading cartridge
        Llúcia and Paula

Break

11:00 – 13:30 Loading libreries for sequencing
        Llúcia and Paula

13:30 – 15:00 Course feedback and wrap up
        Llúcia and Paula