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Summer School Director: 
M. Pilar Francino
 
Summer School Administrator:
Kevin Cifuentes
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SUMMER SCHOOL - Module 2

 

 

 


Module 2/ Preparation of Genomic and Amplicon Libraries for Illumina Platforms.

Dates: July 4th- July 6rd 2018

Coordinator: Llúcia Martínez Priego (martinez_lucpri@gva.es)

The Sequencing Service of Fundació per al Foment de la Investigació Sanitària i Biomèdica de la Generalitat Valenciana (FISABIO – Salut Pública) proposes a basic course in preparation of metagenomic (whole genome sequencing) and amplicon libraries (16S and others) for Illumina platforms.

The course is addressed to professionals of microbiology services in hospitals, NGS laboratory technicians and other professionals in microbiology, health sciences and related fields who are interested in learning new techniques to approach the study of the microbiome.

The module will be practical and hands-on, and will teach participants to prepare two different libraries for Illumina platforms: 16S amplicon libraries for taxonomy analyses and whole genome amplification libraries for metagenomic analyses.

Brief theoretical sessions and practical hands-on sessions will be taught by FISABIO Sequencing Service staff.

Official language: English or Spanish (depending on the students’ origin)

PREREGISTRATION

Module costs: 480 euros

Pre-registration (not open) until 15th of April 2018
Registration deadline 31st of May 2018


Instructors:

Llúcia Martínez Priego PhD. Manager in the Sequencing Service of FISABIO-SP.
Griselda de Marco. MS. Research Assistant in Sequencing Service of FISABIO-SP.

 

Llúcia Martínez Priego

PhD. Manager in the Sequencing Service of FISABIO-SP.

martinez_lucpri@gva.es

Griselda de Marco

MS. Research Assistant in Sequencing Service of FISABIO-SP.

 




Course program 2018:

Wednesday July 4th:


09:15 – 10:00 An Introduction to NGS library preparation
        Llúcia Martínez Priego

10:00 – 10:30 Best practices in a NGS laboratory
        Llúcia Martínez Priego

Break

11:00 – 12:00 DNA quantification and normalisation
        Llúcia and Paula

12:00 – 13:00 16S amplicon PCR
        Llúcia and Griselda

Lunch

14:30 – 16:30 DNA tagmentation and library clean up
        Llúcia and Griselda

16:30 – 17:30 Indexing
        Llúcia and Griselda


Thursday July 5th:

09:00 – 10:00 Indexed library clean up
        Llúcia and Griselda

10:00 – 10:30 Bioanalyzer set up for library validation    
        Llúcia and Griselda

Break

11:00 – 11:30 Library quantification
        Llúcia and Griselda

11:30 – 13:00 Review of library validation results
        Llúcia Martínez Priego

Lunch

14:30 – 16:30 Library normalization
        Llúcia Martínez Priego

16:30 – 17:30 Create Sample sheet for Illumina MiSeq RUN
          Llúcia Martínez Priego

Friday July 6th:

09:00 – 10:45 Preparing the MiSeq and the loading cartridge
        Llúcia Martínez Priego

Break

11:00 – 13:30 Loading libreries for sequencing
        Llúcia Martínez Priego

13:30 – 15:00 Course feedback and wrap up
        Llúcia Martínez Priego

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