|SUMMER SCHOOL - Module 1|
Module 1/ Preparation of Metagenomic and Amplicon Libraries for Illumina Platforms.
Coordinator: Llúcia Martínez Priego (firstname.lastname@example.org)
NEW EDITION IN SEPTEMBER!
Dates: September 27th – September 29th 2017
The course is addressed to professionals of microbiology services in hospitals, NGS laboratory technicians and other professionals in microbiology, health sciences and related fields who are interested in learning new techniques to approach the study of microbiota.
The module will be practical and hands-on, and will teach participants to prepare two different libraries for Illumina platforms: 16S amplicon libraries for taxonomy analyses and whole genome amplification libraries for metagenomic analyses. Brief theoretical sessions and practical hands-on sessions will be taught by FISABIO Sequencing Service staff.
Module costs: 480 euros
Llúcia Martínez Priego PhD. Manager in the Sequencing Service of FISABIO-SP.
Wednesday September 27th:
10:00 – 10:30 Best practices in a NGS laboratory
11:00 – 12:00 DNA quantification and normalisation
12:00 – 13:00 16S amplicon PCR
14:30 – 16:30 DNA tagmentation and library clean up
16:30 – 17:30 Indexing
10:00 – 10:30 Bioanalyzer set up for library validation
11:00 – 11:30 Library quantification
11:30 – 13:00 Review of library validation results
14:30 – 16:30 Library normalization
16:30 – 17:30 Create Sample sheet for Illumina MiSeq RUN
11:00 – 13:30 Loading libreries for sequencing
13:30 – 15:00 Course feedback and wrap up